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Developing 3D brain organoids to model perinatal brain injury

Description 
We are developing 3-dimensional human brain organoids using induced pluripotent stem cells (iPSCs). We can model the effect of neuroinflammation in our brain organoids, thereby creating an in vitro model of perinatal brain injury. We will use this in vitro 3D model to test the mechanism of action of umbilical cord blood and mesenchymal stem cells, specifically assessing the paracrine and direct effects and determine the optimum stem cell type for reducing neuroinflammation. This project will involve extensive cell culturing with both iPSCs and perinatal stem cells, multicolour flow cytometry and molecular analysis using PCR and protein assays.
Essential criteria: 
Minimum entry requirements can be found here: https://www.monash.edu/admissions/entry-requirements/minimum
Keywords 
perinatal brain injury, organoids, neuroinflammation, 3D model
School 
School of Clinical Sciences at Monash Health / Hudson Institute of Medical Research
Available options 
PhD/Doctorate
Honours
BMedSc(Hons)
Time commitment 
Full-time
Top-up scholarship funding available 
No
Physical location 
Monash Health Translation Precinct (Monash Medical Centre)
Co-supervisors 
Assoc Prof 
Michael Fahey

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